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cloning prepared oligonucleotides  (Addgene inc)


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    Addgene inc cloning prepared oligonucleotides
    Cloning Prepared Oligonucleotides, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 237 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cloning prepared oligonucleotides/product/Addgene inc
    Average 95 stars, based on 237 article reviews
    cloning prepared oligonucleotides - by Bioz Stars, 2026-02
    95/100 stars

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    cloning prepared oligonucleotides  (Addgene inc)
    95
    Addgene inc cloning prepared oligonucleotides
    Cloning Prepared Oligonucleotides, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cloning prepared oligonucleotides/product/Addgene inc
    Average 95 stars, based on 1 article reviews
    cloning prepared oligonucleotides - by Bioz Stars, 2026-02
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    lentivirus preparation short hairpin rna shrna oligos targeting mouse nrf2  (OriGene)
    99
    OriGene lentivirus preparation short hairpin rna shrna oligos targeting mouse nrf2
    TGR5−/− BDL mice showed increased oxidative stress and pro-inflammatory response. WT or TGR5−/− mice underwent BDL. (A) MDA and CAT activities in liver tissues on Day 10 (n=3 per group); (B) TNF-α and IL-6 level on Day 1, 3, 7, and 10 measured by ELISA (n=3 per group); (C) TGR5, TLR4, and HO-1 expression in liver on Day 10 (n=3 per group); (D) the expression levels of NF-κB (nuclear and cytoplasmic) and <t>Nrf2</t> (nuclear and cytoplasmic) in liver on day 10 (n=3 per group). **P<0.01, ***P<0.001 (vs. control); #P<0.05, ##P<0.01 (vs. WT). BDL, bile duct ligation. MDA, malondialdehyde; CAT, catalase.
    Lentivirus Preparation Short Hairpin Rna Shrna Oligos Targeting Mouse Nrf2, supplied by OriGene, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lentivirus preparation short hairpin rna shrna oligos targeting mouse nrf2/product/OriGene
    Average 99 stars, based on 1 article reviews
    lentivirus preparation short hairpin rna shrna oligos targeting mouse nrf2 - by Bioz Stars, 2026-02
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    TGR5−/− BDL mice showed increased oxidative stress and pro-inflammatory response. WT or TGR5−/− mice underwent BDL. (A) MDA and CAT activities in liver tissues on Day 10 (n=3 per group); (B) TNF-α and IL-6 level on Day 1, 3, 7, and 10 measured by ELISA (n=3 per group); (C) TGR5, TLR4, and HO-1 expression in liver on Day 10 (n=3 per group); (D) the expression levels of NF-κB (nuclear and cytoplasmic) and Nrf2 (nuclear and cytoplasmic) in liver on day 10 (n=3 per group). **P<0.01, ***P<0.001 (vs. control); #P<0.05, ##P<0.01 (vs. WT). BDL, bile duct ligation. MDA, malondialdehyde; CAT, catalase.

    Journal: Annals of Translational Medicine

    Article Title: TGR5 protects against cholestatic liver disease via suppressing the NF-κB pathway and activating the Nrf2/HO-1 pathway

    doi: 10.21037/atm-21-2631

    Figure Lengend Snippet: TGR5−/− BDL mice showed increased oxidative stress and pro-inflammatory response. WT or TGR5−/− mice underwent BDL. (A) MDA and CAT activities in liver tissues on Day 10 (n=3 per group); (B) TNF-α and IL-6 level on Day 1, 3, 7, and 10 measured by ELISA (n=3 per group); (C) TGR5, TLR4, and HO-1 expression in liver on Day 10 (n=3 per group); (D) the expression levels of NF-κB (nuclear and cytoplasmic) and Nrf2 (nuclear and cytoplasmic) in liver on day 10 (n=3 per group). **P<0.01, ***P<0.001 (vs. control); #P<0.05, ##P<0.01 (vs. WT). BDL, bile duct ligation. MDA, malondialdehyde; CAT, catalase.

    Article Snippet: Lentivirus preparation Short hairpin RNA (shRNA) oligos targeting mouse Nrf2 ( Table S1 ) were cloned into pLKO.1 vector (OriGene, Rockville, MD).

    Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Control, Ligation

    TGR5 agonist INT-777 inhibited ROS production, pro-inflammatory response, and M1-predominant polarization in vitro. Primary Kupffer cells from BDL mice were administered INT-777 (3 µM) for 24 hours. (A) ROS levels; (B) mRNA levels of iNOS2 and Arg I; (C) TNF-α and IL-6 secretion; (D) protein levels of TLR4 and HO-1; (E) protein levels of NF-κB (nuclear and cytosol) and Nrf2 (nuclear and cytoplasm). **P<0.01, ***P<0.001 (vs. WT-vehicle); #P<0.05, ##P<0.01, ###P<0.001 (vs. WT-INT-777). ROS, reactive oxygen species.

    Journal: Annals of Translational Medicine

    Article Title: TGR5 protects against cholestatic liver disease via suppressing the NF-κB pathway and activating the Nrf2/HO-1 pathway

    doi: 10.21037/atm-21-2631

    Figure Lengend Snippet: TGR5 agonist INT-777 inhibited ROS production, pro-inflammatory response, and M1-predominant polarization in vitro. Primary Kupffer cells from BDL mice were administered INT-777 (3 µM) for 24 hours. (A) ROS levels; (B) mRNA levels of iNOS2 and Arg I; (C) TNF-α and IL-6 secretion; (D) protein levels of TLR4 and HO-1; (E) protein levels of NF-κB (nuclear and cytosol) and Nrf2 (nuclear and cytoplasm). **P<0.01, ***P<0.001 (vs. WT-vehicle); #P<0.05, ##P<0.01, ###P<0.001 (vs. WT-INT-777). ROS, reactive oxygen species.

    Article Snippet: Lentivirus preparation Short hairpin RNA (shRNA) oligos targeting mouse Nrf2 ( Table S1 ) were cloned into pLKO.1 vector (OriGene, Rockville, MD).

    Techniques: In Vitro

    Nrf2 interference alleviated the effect of INT-777. Nrf2-silencing Kupffer cells were administered with INT-777 (3 µM) for 24 h. (A) ROS levels; (B) mRNA levels of iNOS2 and Arg I; (C) TNF-α and IL-6 secretion; (D) protein levels of TLR4 and HO-1; (E) protein levels of NF-κB (nuclear and cytoplasm). ***P<0.001 (vs. shNC-vehicle); ##P<0.01, ###P<0.001 (vs. shNrf2-vehicle). Nrf2, nuclear factor 2; ROS, reactive oxygen species.

    Journal: Annals of Translational Medicine

    Article Title: TGR5 protects against cholestatic liver disease via suppressing the NF-κB pathway and activating the Nrf2/HO-1 pathway

    doi: 10.21037/atm-21-2631

    Figure Lengend Snippet: Nrf2 interference alleviated the effect of INT-777. Nrf2-silencing Kupffer cells were administered with INT-777 (3 µM) for 24 h. (A) ROS levels; (B) mRNA levels of iNOS2 and Arg I; (C) TNF-α and IL-6 secretion; (D) protein levels of TLR4 and HO-1; (E) protein levels of NF-κB (nuclear and cytoplasm). ***P<0.001 (vs. shNC-vehicle); ##P<0.01, ###P<0.001 (vs. shNrf2-vehicle). Nrf2, nuclear factor 2; ROS, reactive oxygen species.

    Article Snippet: Lentivirus preparation Short hairpin RNA (shRNA) oligos targeting mouse Nrf2 ( Table S1 ) were cloned into pLKO.1 vector (OriGene, Rockville, MD).

    Techniques: